Fig 2. Quantitative analysis of responses of feline bitter receptors to bitter compounds.

HEK293 cells were transiently transfected with a feline bitter receptor (Tas2r2, Tas2r4, Tas2r7, Tas2r12, Tas2r38, Tas2r46, or Tas2r67), with Gα16-gust44, and assayed for their responses to bitter compounds. Data are expressed as mean ± SE percent change in fluorescence (ΔF, peak fluorescence–baseline fluorescence) compared with baseline fluorescence (F) from three independent wells. Two-tailed Student’s t-tests were performed to determine when responses from Tas2r-transfected cells were significantly different from that of mock-transfect cells. Only bitter compounds that elicited significant responses above mock-transfected cell baseline are shown in the Figure.