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. 2015 Oct 21;10(10):e0141172. doi: 10.1371/journal.pone.0141172

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© 2015 Cho et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Genes of interest (i.e. HrasV12 or LTg) were inserted in between MSCV LTRs, and either GFP or RFP gene was used as a tracer gene. (B) Inserts cloned into pMSCV plasmids were confirmed by enzymatic digestions with either BamHI or EcoRI. M: DNA ladder, 1: pMSCV-GFP; 2: pMSCV-HrasV12-GFP; 3: pMSCV-GFP^cut; 4: pMSCV-HrasV12-GFP^cut; 5:pBABE-HrasV12^cut (+ control); 6: pMSCV-RFP; 7: pMSCV-SV40 LTg-RFP; 8: pMSCV-RFP^cut 9: pMSCV-SV40 LTg-RFP^cut; 10: pBABE-SV40 LTg^cut (+ control). White arrows indicate inserts. Sequences of insert were also verified by DNA sequencing.