Fig. 5.

Semi-quantitative RT-PCR analysis of the top sex-biased PERNI transcripts. Semi-quantitative RT-PCR analysis of selected transcripts of male and female adults of P. perniciosus. a Normalization control using primer pairs for the sod gene of P. perniciosus at 20, 25 and 30 PCR amplification cycles. b Amplification of female-biased PERNI transcripts. For the transcript F4 we observed an extra male-specific amplification signal of larger molecular size. c Amplification of male-biased PERNI transcripts. For the transcript M1 we observed an extra male-specific amplification signal of larger molecular size and for the transcripts M5a, M6, M8, M15, M17 and M19 we observed extra female-specific amplification signals of larger molecular size. For the transcript M9 we observed an extra non-sex-specific amplification signals of larger molecular size. λ indicates the molecular weight marker (λ genomic DNA EcoRI-HindIII digested)