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. 2015 Oct 24;14:96. doi: 10.1186/s12938-015-0093-6

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© Sheikhzadeh et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Image processing pipeline of confocal images using MATLAB algorithm. a Raw confocal image of a normal area; b edge detection by Canny algorithm; c nuclei segmentation; and d creation of nuclei masks followed by extraction of their centers of gravity