Table 3.

Variants identified in corresponding single colonies derived from different clinical isolates

	Single colony 1			Single colony 2			Single colony 3
	Totala	Fixedb	Heteroc	Totala	Fixedb	Heteroc	Totala	Fixedb	Heteroc	Total variation
R160	5	2	3	4	3	1	-	-	-	9
R376	3	1	2	8	2	6	6	1	5	17
R458	3	3	0	5	2	3	3	0	3	11
R486	0	0	0	2	0	2	-	-	-	2
R631	5	0	5	6	0	6	8	0	8	19
R637	0	0	0	0	0	0	0	0	0	0
R641	9	9	0	3	0	3	6	0	6	18
R721	0	0	0	4	0	4	0	0	0	4
R912	4	4	0	6	3	3	7	7	0	17
R965	4	4	0	1	1	0	-	-	-	5
R966	5	0	5	1	0	1	6	0	6	12
R1035	0	0	0	0	0	0	0	0	0	0
R1415	0	0	0	0	0	0	0	0	0	0

^aTotal number variants unique between the corresponding single colonies

^bFixed variants as defined as having a read frequency of ≥ 70 %

^cHeterogeneous variants as defined as having a read frequency < 70 % and ≥ 30 %

-A third single colony was not available for the comparison