Fig. 4.

PJ-34 causes DNA damage and p53 pathway activation, even in MT-2 and C91PL cells. PJ-34 induces cell apoptosis in most of HTLV-1-transformed and ATL cells except for C91PL and MT-2 cells. a PBMCs, HTLV-1-transformed (C8166, MT-2, C91PL, MT4), and (b) ATL cells (ATL-MT-1, ATL-ED, ATL-KOB, ATL-T, ATL-43T, ATL-25, ATL-55T) were treated with DMSO or different concentrations of PJ-34 (5, 10, 25, 50 μM) for 5 days. Cells were subsequently stained for annexin V and PI and analyzed for apoptosis by FACS analysis. Percentage of increased apoptotic and necrotic cells in HTLV-1-transformed cells and ATL cell treatment with different amounts of PJ-34 were graphed