Fig. 8.

The expressions of known resistant markers in MT-2 and sensitive cell lines. (The defect of caspase 3 activation in MT-2 and C91PL contributes to the resistance of PJ-34 in MT-2 and C91PL cells.) a, b Relative expression of p53BP1 (a) and BRCA1 (b) was compared using real-time RT-PCR among resistant cell lines MT-2, C91PL, and other sensitive cell lines. Real-time PCR was performed in duplicate, and data were normalized to GAPDH expression. Results were presented as mean ± SD from two independent experiments. c Relative expression of p53BP1, p65/RELA, BRCA1, and PARP-1 was compared using real-time RT-PCR among MT-4, C8166, and MT-2 treated with DMSO or 25 μM of PJ-34 for 5 days. Real-time PCR was performed in duplicate, and data were normalized to GAPDH expression