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. 2015 Oct 24;15:38. doi: 10.1186/s12861-015-0086-5

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© Freyer et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Expression of Venus at somite stages. a Frontal and lateral views of wholemount Gata6 ^H2B-Venus/+ embryos merged with brightfield images at E8.25 (4 somite stage, ss). Venus is expressed in the heart, sinus venosus, and gut endoderm. b Lateral views of wholemount Venus expression at E9.5 (23ss) in Gata6 ^H2B-Venus/+ embryos. c Immunofluorescence for GATA6 (red) on transverse sections through a Gata6 ^H2B-Venus/+ embryo. Varying levels of GATA6 co-expression with Venus (green) can be seen in the gut endoderm and heart. Some tissues express GATA6 exclusively, for example the body wall and endocardium, while other tissues express Venus only, such as the yolk sac and weak expression in the pharyngeal mesoderm. Nuclei are stained with Hoechst (blue)