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. 2015 Oct 24;15:254. doi: 10.1186/s12870-015-0640-0

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© Pascual et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Functional analysis of the PpNAC3 promoter in VIGS-silenced NbbHLH2 plants. a Photographs of 6-week-old control (pTRV-control) and NbbHLH2-silenced plants. b The relative expression level of NbbHLH2 was determined by qPCR in pTRV control and N. benthamiana plants with NbbHLH2 suppression. c GFP expression driven by the PpNAC3 promoter in pTRV-NbbHLH2-silenced plants. After 48 h of agroinfiltration with the P_NAC3-GFP construct, the leaves were treated with either 100 μM MeJA or with water as a control. NbActin was used as the endogenous qPCR control. The error bars represent the SD from three biological replicates measured in triplicate