Skip to main content
. 2015 Oct 21;10(10):e0139882. doi: 10.1371/journal.pone.0139882

Fig 3. Preparative gel filtration of wild type and F119L phosphomannomutase2.

Fig 3

wt-PMM2 and F119L, alone and in the presence of R141H were fractionated on a Superdex75 column equilibrated in Hepes 20 mM pH 7.5, NaCl 150 mM, MgCl2 1 mM. The fractions were analyzed for the protein content and for the phosphoglucomutase activity (in the presence of 40 μM Glc-1P and 5 μM of Glc-1,6-P2). Before loading, each protein sample was pretreated with EDTA on ice. Panel A and B: F119L and F119L+R141H (1:1 and 1:3.5). Panel C and D: wt-PMM2 and wt-PMM2+R141H (1:4).