Fig 1. Yeast growth inhibition assay.
The growth of yeast strain BY4741 expressing wild type and mutant proteins of VopK under the control of GAL1 promoter from pESC-LEU (A) or pGMH10 (B). Cells transformed with empty vector alone were used as a negative control. The indicated strains were grown overnight in non-inducing selective synthetic medium (SCraf) containing 2% raffinose as the carbon source. Equal number of cells were spotted at 10° and three serial dilutions of 10−2, 10−3 and 10−4 (left to right) on SCGlu (left panel, repression) and SCGal (induction) agar. The data shown are representative of three independent experiments.