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. 2015 Jul 10;99(21):9037–9048. doi: 10.1007/s00253-015-6778-8

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© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 7 — Extracellular production of phosphorylated S. aureus IsdB in L. lactis. a Cytosolic (C) and growth medium (M) fractions of L. lactis PA1001 producing His₆-IsdB (4110) and His₆-TEV-IsdB (4111) were analysed by LDS-PAGE and stained with SimplyBlue SafeStain (SB) or Pro-Q Diamond staining (Pro-Q). As a control, Western blotting was performed, using anti-His₆ antibodies (α-His). The arrow marks the position of mature IsdB, and the positions of Mw marker proteins are indicated