Skip to main content

View full-text article in PMC

. 2015 Jul 10;99(21):9037–9048. doi: 10.1007/s00253-015-6778-8

Table 2.

Primers used for the construction of the expression vectors

Primer	5′ → 3′ nucleotide sequence^a	Restriction site^b
NHis_F	TATGCACCATCACCATCACCATGGATCCGAATTCCTCGAGGCGGCCGCATAAA
NHis_R	ACGTGGTAGTGGTAGTGGTACCTAGGCTTAAGGAGCTCCGCCGGCGTATTTCTAG
CHis_F	TATGGGATCCGAATTCCTCGAGGCGGCCGCACACCATCACCATCACCATTAAA
CHis_R	ACCCTAGGCTTAAGGAGCTCCGCCGGCGTGTGGTAGTGGTAGTGGTAATTTCTAG
NHisTEV_F	TATGCACCATCACCATCACCATGAGAACCTGTACTTCCAGGGATCCGAATTCCTCGAGGCGGCCGCATAAA
NHisTEV_R	ACGTGGTAGTGGTAGTGGTACTCTTGGACATGAAGGTCCCTAGGCTTAAGGAGCTCCGCCGGCGTATTTCTAG
pEF110.f	CCCCGTCTC CCATGCACCATCACCATCATCATGGATCCGAA	BsmBI
pEF110.r	CCCAAGCTT TTA CCATGGTGCGGCCGCCTCGAGG	HindIII and NotI
pEF111.f	CCCCGTCTC CCATGCACCATCACCATCACCATGAG	BsmBI
pEF111.r	CCCAAGCTT TTATGCGGCCGCCTCGAGG	HindIII
pEF210.f	CCCCCATGGGATCCGAATTCCTCGAGGC	NcoI
pEF210.r	CCCAAGCTT TTAATGGTGATGGTGATGGTGTGC	HindIII

^aRestriction site underlined, stop codon in bold

^b BsmBI restriction resulting in NcoI overhang in italic