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. 2015 Oct 24;13:307. doi: 10.1186/s12957-015-0717-0

Fig. 1.

Fig. 1

Low level of Klotho is detected in the liver cancer cell lines. The liver cancer cells (5 × 105 cells/well) were plated into 6-well plate. After 8 h, the endogenous Klotho levels in a panel of four liver cancer cell lines were detected with RT-PCR (a) and western blotting analysis (b). L02 is an immortalized liver cell line, and GAPDH is used as an internal reference