Fig. 2.

Overexpression or recombinant Klotho administration suppresses the proliferation of liver cancer cells. a The liver cancer cells (2 × 10⁴ cells/per well) were plated into 48-well plate. After 8 h, the cells were transfected with p CMV6-Klotho and control vector for 24, 48, 72, and 96 h. The cells transfected by p CMV6 vector were used as the controls. Data are shown as means ± SD. **p < 0.01 compared with control cells. b The liver cancer cells HepG2 and SMMC-7721 (1 × 10³ cells/per well) were plated into 96-well plate. After 8 h, the cells were treated with recombinated Klotho protein and control protein BSA for 24, 48, 72, and 96 h. Data are shown as means ± SD. **p < 0.01 compared with the cells treated with BSA