Fig. 4.

Klotho expression induces cell apoptosis in human liver cancer cells. a The HepG2 cells were plated into 6-well plate and were transfected with pCMV6-Klotho and pCMV6 vector for 48 h. Then, the cells were fixed, dispersed, and stained with Annexin V and PI. The cell apoptosis rate was detected by flow cytometry analysis. The histogram of HepG2 (b) and SMMC-7721 (c) were shown. The cells transfected with pCMV6 vector were used as negative controls. **p < 0.01, compared with negative control