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. 2015 Oct 23;11:269. doi: 10.1186/s12917-015-0579-2

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© Lee et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Survival rates of zebrafish inoculated with different dosage levels of Edwardsiella tarda CK41 as time progressed. E. tarda CK41 cells from fresh culture were administrated to four groups of zebrafish (n = 20 in each group) at doses of 7.2 × 10⁸ CFU/200 ml (◆), 7.2 × 10⁹ CFU/200 ml (■), 7.2 × 10¹⁰ CFU/200 ml (▲), and 7.2 × 10¹¹ CFU/200 ml (●) by immersion route. Control zebrafish were inoculated with only PBS 200 ml (×). Mortality observations were recorded daily for an additional 14 days post-infection. Dose was determined by viable counting of bacterial cells after administration