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. 2015 Oct 21;10(10):e0140868. doi: 10.1371/journal.pone.0140868

Fig 1. Specificity of in-house VAMP2 antibody.

Fig 1

(A) Brain homogenates from wild type (WT1–2) and knockout (KO1–3) mice. The VAMP2 band at 18kDa is absent from brains of the three knockout mice. Protein loaded 30 μg. (B) Western blots from wild type rat brain. BH: brain homogenate; S: crude synaptosomes; SV: synaptic vesicles. Only one band was seen in each preparation. Protein loaded 10 μg. (C) Quantitative analysis of immunogold labeling with anti-VAMP2 of presynaptic cytoplasm at excitatory synapses in hippocampus, preincubated with VAMP2 protein. Ultrastructural quantification of VAMP2 immunogold labeling of presynaptic cytoplasm at excitatory synapses in hippocampus of VAMP2 KO mice. Statistical significance (p < 0.001). (D) Electron micrographs showing labeling for VAMP2. (D1) Standard labeling, i.e. normal immunogold without pre-incubation with antigen. (D2) Pre-incubation with VAMP2 antigen. (D3) Standard labeling of wildtype. (D4) Labeling of VAMP2-KO tissue. Scale bar: 125 nm.