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. 2015 Oct 21;10(10):e0141077. doi: 10.1371/journal.pone.0141077

Fig 3. Immunofluorescent staining of occludin (in red) in control and in inhibitor MI-432-treated IPEC-J2 cells after different treatment times.

Fig 3

The cells were not completely differentiated when the inhibitor administration (50 μM) was started. Occludin can be seen in red and cell nuclei were stained with DAPI in blue. MI-432-induced matriptase suppression resulted in occludin loss in TJ strands. White arrows indicate the characteristic membranous pattern of occludin presence in the control panel.