Table 6.
The difference between normalizers
| miRNAs | Internal controls | |||
|---|---|---|---|---|
| U6 | miR-16 | U91 | U6 + U91 | |
| miR-21 | 2.97E-06; p < 0.001 | 0.003404; p < 0.01 | 0.7979; p > 0.05 | 0.000789; p < 0.01 |
| miR-96 | 0.003078; p < 0.01 | 0.02723; p < 0.05 | 0.639203; p > 0.05 | 0.038631; p < 0.05 |
| miR-148a | 4.66E-06; p < 0.001 | 0.007849; p < 0.01 | 0.3078; p > 0.05 | 0.000586; p < 0.01 |
| miR-155 | 6.13E-05; p < 0.01 | 0.000873; p < 0.01 | 0.534202; p > 0.05 | 0.001831; p < 0.01 |
| miR-196a | 7.93E-07; p < 0.001 | 0.042034; p < 0.05 | 0.862491; p > 0.05 | 0.000117; p < 0.01 |
| miR-217 | 0.003951; p < 0.01 | 0.065696; p > 0.05 | 0.024668; p < 0.05 | 0.008373; p < 0.05 |
MicroRNA expression levels were normalized relative to the alien spike, in comparison with the normalizations relative to other internal controls. Data are presented as P-values of the paired Student’s t-test. p < 0.05 was considered statistically significant. P-values for the statistically significant differences are shown in bold