Table 3.
CDR length distribution of the pre and post-selection sequences
| A | CDR length (percentage of representation within the pre-selection sequences) | |||||
|---|---|---|---|---|---|---|
| CDR H1 | CDR H2 | CDR H3 | CDR L1 | CDR L2 | CDR L3 | |
| Library 1 | 5 (100) | 16 (97) | 14 (14.5) | 14 (65) | 7 (100) | 10 (62.5) |
| 18 (2) | 13 (11.8) | 11 (20) | 11 (28.8) | |||
| 12 (10) | 13 (14) | 9 (2.5) | ||||
| 11 (8.18) | ||||||
| Library 2 | 5 (96.1) | 16 (98.7) | 11 (18) | 14 (67) | 7 (100) | 10 (56.7) |
| 6 (2.6) | 15 (1.3) | 14 (16) | 13 (20) | 11 (30) | ||
| 7 (1.3) | 13 (12) | 11 (13.3) | 12 (13.3) | |||
| 9 (1.3) | 9 (0) | |||||
| Library 3 | 5 (100) | 16 (100) | 13 (16) | 14 (52.6) | 7 (100) | 10 (67.4) |
| 14 (15) | 13 (24.2) | 11 (21.1) | ||||
| 15 (13) | 11 (20) | 12 (7.4) | ||||
| 12 (8.7) | ||||||
| B | CDR length (percentage of representation within the post-selection clones) | |||||
| CDR H1 | CDR H2 | CDR H3 | CDR L1 | CDR L2 | CDR L3 | |
| Library 1 | 5 (100) | 16 (100) | 12 (89) | 14 (89) | 7 (100) | 11 (89) |
| 11 (11) | 11 (11) | 10 (11) | ||||
| Library 2 | 5 (100) | 16 (100) | 11 (50) | 13 (75) | 7 (100) | 9 (50) |
| 9 (25) | 14 (25) | 10 (50) | ||||
| 13 (25) | ||||||
| Library 3 | 5 (100) | 16 (100) | 12 (100) | 13 (100) | 7 (100) | 11 (100) |
The most prevalent CDRs lengths (number of amino acids) within each library in (A) Pre-selection clones and (B) Post-selection clones. The percentage of each specific CDR length within (A) the entire library population or (B) post-selection clones is in parenthesis