Table 4.
Canonical combinations of the pre and post-selection sequences
| A | Canonical combination (percentage of representation within the pre-selection sequences) | |
|---|---|---|
| CDRs H1-H2 | CDRs L1-L2-L3 | |
| Library 1 | 1-1 (97.3) | 6-1-X (51.3) |
| 2-4 (1) | 2-1-5 (11.2) | |
| 1-4 (1) | 6-1-5 (11.2) | |
| 2-1-X (10) | ||
| Library 2 | 1-1 (94.8) | 6-1-X (46.7) |
| 2-1 (2.6) | 6-1-5 (20) | |
| 3-1 (1.3) | 5-1-X (16.6) | |
| 5-1-4 (−) | ||
| Library 3 | 1-1 (100) | 6-1-X (45.3) |
| 5-1-X (14.7) | ||
| 2-1-X (17.7) | ||
| 5-1-5 (5.26) | ||
| B | Canonical combination (percentage of representation within the post-selection clones) | |
| CDRs H1-H2 | CDRs L1-L2-L3 | |
| Library 1 | 1-1 (100) | 6-1-5 (88.9) |
| 2-1-X (11.1) | ||
| Library 2 | 1-1 (100) | 6-1-X (25) |
| 5-1-4 (50) | ||
| 5-1-X (25) | ||
| Library 3 | 1-1 (100) | 5-1-5 (100) |
The canonical classes were determined using Strict Chothia SDR template [7, 9, 53, 63]. Data presented represent the most prevalent canonical combinations within each library in (A) Pre-selection clones and (B) Post-selection clones. X: indicates no currently recognized canonical class with the same loop length. The percentage of specific canonical combination within the entire library population of the analyzed pre (A) and post (B)-selection sequences are in parenthesis