Fig 4. T = 1 SVPs of GNNV.

(A) Surface presentations of the monomers engaged in pentameric interactions in the T = 3 and T = 1 GNNV capsids. Each iASU is viewed along the I5 axis and shown in green, wheat, cyan, yellow and magenta colors, respectively. The diameters of three capsids are indicated. (B) A superimposition of the calcium-binding regions of the S-domain between the T = 3 GNNV-LP and the T = 1 delta-P-domain mutant. Gln100, Asp130, Asp133, Ser170 and Glu213, which participate in the Ca²⁺ coordination in the T = 3 GNNV-LP (yellow), exhibit conformational changes in the T = 1 delta-P-domain mutant (green). The hydrogen bonds are indicated with blue dotted lines. (C) A comparison of the spatial relationship of subunit pairs in the T = 3 GNNV-LP and the T = 1 delta-P-domain mutant. Subunit packing and the nomenclature of the T = 3 and the T = 1 capsids are shown. Rotation angles (°) and translation distances (Å) are identified and compared between different subunit pairs of the T = 3 and the T = 1 GNNV capsids.