Fig 2. Identification of ManNAz in the juxtanuclear region of the cell and in secretory granules.
Cells were labeled for 24 h with ManNAz and afterwards immunolabeled with anti-CD63. For confocal microscopy, ManNAz was coupled to Alexa 488 and anti-CD63 was detected with secondary antibody conjugated to Alexa 594. (Bars: 10μm).