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. 2015 Oct 22;10(10):e0139888. doi: 10.1371/journal.pone.0139888

Fig 6. Knockdown of PLD2 in RBL-2H3 cells results in a phenotype similar to PLD2CI cells.

Fig 6

(A) Cells were pulsed for 1 h with ManNAz and chased for 1 and 3 h (Bars: 10μm). (B) RBL-2H3 cells transfected with PLD2 siRNA had a 65% reduction in PLD2 expression when compared to control siRNA cells. P = 0.0279. (C) The fluorescence intensity of ManNAz in PLD2 siRNA cells was significantly lower than in control siRNA cells after 3 h of chase. P = 0.014 for 3 hours. For confocal microscopy, ManNAz was coupled to Alexa 488.