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. 2015 Oct 22;10(10):e0141370. doi: 10.1371/journal.pone.0141370

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© 2015 Ranganathan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 10 — A. In soft agar assay, colonies formed in top agar layer by cancer cells after 20 days incubation were stained with 0.005% crystal violet and counted under a light microscope. B. The number of colonies formed after treatment with quercetin compared with those formed by twist/MCF-7 cells is shown at the graph and the twist/MCF-7 compared with control MCF-7 which are statistically significant ‘*‘ as determined by Student’s t-test. The significant values represent mean ± SD of three separate experiments, each consisting of duplicate cultures. Significance is determined as *p<0.05.