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. 2015 Oct 22;11(10):e1005559. doi: 10.1371/journal.pgen.1005559

Fig 1. Characterization of the asi3-1 (met18-3) mutant.

Fig 1

A. Root phenotypes of the asi3-1 mutant on ½ MS medium containing 1% glucose or sucrose. Seedlings were photographed 14 days after germination. Col-0, 35S::SUC2, and ros1-13 were used as controls. B. Quantitative RT-PCR results showing the relative expression levels of SUC2, NPTII, and HPTII transgenes in asi3-1 mutant and 35S::SUC2 seedlings. ACTIN 2 was used as an internal control. C. Kanamycin-sensitivity assay. D. Map-based cloning of the asi3-1 mutation. E. The gene model structure of the AT5G48120 (ASI3/MET18) gene. A Q-to-stop codon mutation in the 142nd amino acid of the AT5G48120 gene is in asi3-1 (met18-3) mutant. Two other T-DNA insertion mutants are shown. The exon, intron, and UTR regions are marked by a black box, black line, and white box, respectively. F. Root phenotypes of the MET18-3FH/asi3-1 transgenic lines. Several randomly selected transgenic plants in the T3 generation were grown on ½ MS medium containing 1% sucrose. Seedlings were photographed 12 days after germination. G. Quantitative RT-PCR results showing the SUC2 transgene expression levels in MET18-3FH transgenic plants.