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. 2015 Oct 22;11(10):e1005559. doi: 10.1371/journal.pgen.1005559

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© 2015 Duan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A. Diagram of the 35S::SUC2 transgene and an IGB snapshot of DNA methylation levels in the promoter region in different cytosine contexts. The positions of primer pairs used in ChIP-qPCR are as labeled. B. DNA methylation levels in the promoter region of 35S::SUC2. DNA methylation levels were quantified based on whole-genome bisulfite sequencing data. C. DNA methylation-sensitive PCR (Chop-PCR) results showing increased DNA methylation in met18-3 and ros1-13 mutants. Complementation with MET18 genomic sequence rescued increased DNA methylation. Non-digested DNA was used as the control PCR template.