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. 2015 Oct 22;11(10):e1005559. doi: 10.1371/journal.pgen.1005559

Fig 6. MET18 physically associates with ROS1.

Fig 6

A. Mass spectrometric analysis of MET18 affinity purification. Selected unique proteins co-purified from MET18-3FH transgenic plants but not from wild-type are indicated. B.Y2H assays of protein interactions between ROS1, MET18 and AE7. MET18-AE7 interaction served as a positive control. C. Split luciferase assay of interactions between ROS1 and MET18 or AE7 in Arabidopsis protoplasts confirmed that ROS1 interacts with MET18 and AE7. ROS1, MET18 and AE7 were transiently expressed in protoplasts by plasmids transfection. D. Split luciferase assay of protein interactions in tobacco leaves. The full-length and deletion forms of ROS1 proteins were designated with white boxes. The four conserved amino acids were designated with red line as iron-sulfur motif (blue dashed line). The photograph was taken at 3 day-post-infiltration.