Table 1. Primer pair information.

Primer name	Primer location	Sequence 5’- 3’	T m [°C]
Betula spp. ITS for	ITS	CGG TAG GGA GAC ACT TGT GC	56
Betula spp. ITS rev	ITS	GTC CCT TTG CAA GGA GAT GG	56
BP8 for	BP8	ACG ATC GAG TTT TCA TCA AAC AAA	60
BP8 rev	BP8	GAC CTT ATT GTC TTC ACG GTC CTT	60

Primer names, sequences, and specific annealing temperatures [°C] are given. Primer pair ITS Betula pendula is self-designed to amplify a multi-copy region, primer pair BP8 after Longhi et al. 2009 amplifies a single-copy gene [6].