Table 3. Comparison of qPCR results with simulation and pollen trap data.
| Filter ID | Sampling period (2010) | ITS, cp m-3 | BP8, cp m-3 | Simulated pollen concentration in, pollen m-3 at | Observed pollen concentration in pollen m-3 | |||
|---|---|---|---|---|---|---|---|---|
| coarse | fine | coarse | fine | Mainz | Löwenstein | Löwenstein | ||
| MZ 324 | 2010-03-16 / 2010-03-23 | 35 | 1 | 0 | 0 | n.a. | n.a. | 0 |
| MZ 325 | 2010-03-23 / 2010-03-30 | 37 | 55 | 1 | 0 | n.a. | n.a. | 0 |
| MZ 326 | 2010-03-30 / 2010-04-06 | 396 | 3 | 9 | 2 | 85 | 67 | 0 |
| MZ 329 | 2010-04-06 / 2010-04-13 | 243384 | 4630 | 879 | 24 | 405 | 309 | 98 |
| MZ 330 | 2010–04013 / 2010-04-20 | 76207 | 255 | 809 | 5 | 649 | 389 | 173 |
| MZ 331 | 2010-04-20 / 2010-04-27 | 19826 | 427 | 69 | 3 | 124 | 283 | 158 |
| MZ 332 | 2010-04-27 / 2010-05-04 | 2203 | 24 | 71 | 0 | n.a. | n.a | 17 |
| MZ 335 | 2010-05-04 / 2010-05-11 | 217 | 11 | 17 | 0 | n.a. | n.a. | 1 |
| MZ 336 | 2010-05-11 / 2010-05-18 | 185 | 13 | 10 | 8 | n.a. | n.a. | 0 |
| MZ 337 | 2010-05-18 / 2010-05-25 | 335 | 12 | 4 | 0 | n.a. | n.a. | 0 |
| MZ 338 | 2010-05-25 / 2010-06-01 | 16 | 5 | 16 | 0 | n.a. | n.a. | 0 |
| MZ 339 | 2010-06-01 / 2010-060-08 | 144 | 50 | 4 | 0 | n.a. | n.a. | 0 |
DNA concentrations (cp m-3) are listed for ITS multi-copy region and BP8 single-copy gene for coarse and fine particle filter samples, respectively. The original measured DNA copies in the coarse particle fractions were divided by three to correct for the tricellular nature of Betula pendula pollen grains. Calculated pollen concentrations with COSMO-ART for Mainz and Löwenstein and measured pollen concentrations in pollen grains m-3 via Burkard sampler in Löwenstein were averaged corresponding to the sampling interval of the appropriate filter sample; n.a.: not available.