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. 2015 Oct 22;10(10):e0141020. doi: 10.1371/journal.pone.0141020

Fig 2. Involvement of mitochondria in cooperative cytotoxic action of rosiglitazone and LA-12.

Fig 2

(a) The release of cytochrome c into the cytoplasm (cytoplasmic fraction) of HCT116 cells pretreated (24 h) with rosiglitazone (RGZ, 50 μM) and subsequently treated (48 h) with LA-12 (0.75 μM), detected by Western blotting after cell fractionation. (b) Cleavage of caspase-9, PARP, Bax protein level (Western blotting), and (c) changes in mitochondrial membrane potential (MMP, flow cytometry) in HCT116 wt and Bax-/- cells treated as in a). (d) The level of Noxa, Bim, Puma, Bid, Bax and Bak protein (Western blotting) in HCT116 wt cells treated as in a). Results are means + S.E.M. or representatives of three independent experiments. Statistical significance: P < 0.05, * versus control, ‡ versus RGZ or Ο versus LA-12, and Δ for wt versus Bax-/- cells.