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. 2015 Oct 22;11(10):e1005570. doi: 10.1371/journal.pgen.1005570

Fig 4. Partial suppression of the ALT telomere phenotypes by atr1 and chk1 deletions.

Fig 4

(A) TRF analysis of the uku70 nar1 chk1Δ and uku70 nar1 atr1Δ double mutants after multiple re-streaks on restrictive medium. The indicated strains were passaged at 30°C on YPD. Each streak represents approximately 25 generations of growth. DNAs from streak 3, 5, and 9 of each strain were isolated, treated with PstI, and analyzed by standard telomere Southern analysis. (B) and (C) Pst1-treated DNA was subjected to in-gel hybridization assay to detect unpaired C-strand (B) and unpaired G-strand telomere DNA (C). (D) DNAs from the indicated strains were subjected to C-circle and G-circle assays.