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. 2015 Oct 22;10(10):e0141074. doi: 10.1371/journal.pone.0141074

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© 2015 Somanchi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (a) Dynamic range for the calcein release assay was determined for neuroblastoma and leukemia tumor targets (K562, SK-N-BE(2), CHP134, CHLA155, IMR32, Jurkat, 721.221 and Nalm 6). The cell lines were stained with Calcein AM, and seeded in 96 well plates to generate maximum release (lysis with 1% TritonX-100) and spontaneous release data. After 4 hours the supernatant fluorescence was measured. The maximum release was normalized to 100% and the spontaneous release is represented as % of maximum. (b) Illustration of calcein release from target cells upon lysis by NK cells following necrosis-like and apoptotic death. (c) Bright-field and fluorescence overlay images of calcein release from CHP 134 cells undergoing necrosis-like death following interaction with NK cells. (d) Bright-field and fluorescent overlay images of calcein release from some K562 cells undergoing apoptotic death following interaction with NK cells. The images were derived from live Nikon Biostation IQ-M videos.