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. 2015 Oct 22;10(10):e0141258. doi: 10.1371/journal.pone.0141258

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© 2015 Anzai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A-F) Immunofluorescence staining for CAV2 in the organ of Corti around apical part of cochlea in 3-week-old Cx26^f/fP0Cre mice with GJB2-associated deafness and in littermate controls. Whole-mount cochleae were fixed and immunolabeled with anti-CAV2 (red). Nuclei were counterstained with DAPI (blue). In contrast to the controls, notable accumulation of CAV2 was observed at the organ of Corti in Cx26^f/fP0Cre cochleae. (G) shows the mean percentage of cells with accumulated CAV2 in control and Cx26^f/fP0Cre cochleae. There was a statistically significant difference between the control and Cx26^f/fP0Cre mice. Values represent the mean ± SE (n = 5 mice). ***P = 3.72 × 10⁻⁵, Student’s t-test.