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HEK-293#19 cells stably expressing LOX-1 were treated with filipin (5μM) and glimepiride (5μM) for 15, 30 and 60 min. Total protein extract (5μg) was loaded as a positive internal control of electrophoretic mobility (Extr.). Anti-V5 antibody was used to detect LOX-1-V5 protein and antibody directed against flotilin to verify equal protein loading.
