Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 23;10(10):e0141270. doi: 10.1371/journal.pone.0141270

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Gioia et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 2 — (A) LOX-1-V5-COS transfected cells were treated with filipin (5μM) and glimepiride (5μM) for 15, 30 and 60 min. Conditioned media were centrifuged at 100,000 g and the resulting pellets (P100, lanes 2–4) and supernatants (S100, lanes 5–13) were analyzed by Western blotting. Lane 1 (Extr.) shows total protein extract (5 μg) loaded as a positive internal control of electrophoretic mobility of full-length LOX-1 receptor. (B) Upper panel shows sLOX-1 amount released in cells treated with Filipin (5μM) and MβCD (5mM) for 30 min compared to sLOX-1 constitutively released from untreated cells at 1, 2 and 5 hours. Lower panel shows the densitometric analysis for sLOX-1 band intensity. Data in histograms represent the average ± SEM of three experiments, P < 0.05 (*), P < 0.01 (**) or P < 0.001 (***).