Fig 5. sLOX-1 release in EA.hy926 cells.

(A) EA.hy926 cells were grown overnight in serum free medium in the absence (lanes 1–4) or in the presence (lanes 5–8) of ox-LDL (20μg/ml). MβCD was added to cells for the last 30 min incubation (lane 2, 3 and 8). GM6001 inhibitor was present for 30 min (lane 3) or overnight (lane 6). Western blot analysis of conditioned media was performed with anti-human LOX-1 receptor. (B) MTS assay performed on EA.hy926 cells plated at a density of 1,5x10⁴ cells/well and incubated overnight with different concentration of ox-LDL. Results are expressed as percentage of absorbance ± SEM compared to control cells.