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. 2015 Oct 23;11(10):e1005551. doi: 10.1371/journal.pgen.1005551

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© 2015 Ramkumar et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Western blot of V5-tagged CRUMBS2 expressed in WT or Poglut1 ^wsnp (ws) EBs after being subjected to PNGase F or Endo H digestions (Materials and Methods). EBs were differentiated for 5–6 days. Wild-type CRUMBS2 protein is modified with N-glycans sensitive to PNGase F, whereas CRUMBS2 from Poglut1 ^wsnp EBs is modified with Endo H-sensitive N-glycans, consistent with trapping of the protein in the ER. (B-E) Immunolocalization of CRUMBS2 in transverse sections of E8.5 wild-type neural plate (B) and primitive streak (C). CRUMBS2 in transverse sections of the E8.5 Poglut1 ^wsnp neural plate (D) primitive streak (E) is not localized to the apical cell surface (up). Scale bars: 20 μm.