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. 2015 Oct 23;11(10):e1005569. doi: 10.1371/journal.pgen.1005569

Fig 8. Spermatogonial stem cell (SSCs) transplants reveal Sertoli cell and germ cell defects in Poc1a cha/cha mice.

Fig 8

A. Testis sections from 3 mo wild type and Poc1a cha/cha mice were immunostained for SOX9, a Sertoli cell marker, and PLZF, an undifferentiated spermatogonial marker, and counterstained with DAPI to visualize nuclei (N = 3 animals of each genotype, scale bar = 50 μm). B. Testes from busulfan treated wild-type recipient or Poc1a cha/cha mutant recipients ~3 months after transplantation with wild-type LacZ labeled SSCs. The testes (N = 8) of recipient mice (N = 4) were transplanted for each genotype. C. Testes from busulfan-treated wild-type nude recipient mice ~3 months after transplantation with wild type LacZ-labeled SSCs or Poc1a cha/cha SSCs labeled with LacZ. For both B and C, blue stained areas in recipient testes represent colonies of donor-derived spermatogenesis generated from engrafted SSCs. D. Quantification of γH2AX-positive cells in seminiferous tubules of 3 wild type and 3 Poc1a cha/cha testes at P7.