Table 1. Taqman primers and probes used.
| Gene | Primers and Probe (5'-3') | Application Efficiencies (%) | Product Length (bp) | Reference/ Accession no. |
|---|---|---|---|---|
| IL-1β | (f) TGCTGGTTTCCATCTCGTATGTAC
(r) CCCAGAGCGGCTATTCCA (p) AGTACAACCCCTGCTGCCCCGC (VIC/MGB) |
95 | 80 | NC_006096.3 |
| IL-6 | (f) CCCGCTTCTGACTGTGTTT
(r) GCCGGTTTTGAAGTTAATCTTTT (p) TGTGTTTCGGAGTGCTTT (VIC/MGB) |
86 | 139 | NC_006089.3 |
| TNF-α | (f) CCCCTACCCTGTCCCACAA
(r) ACTGCGGAGGGTTCATTCC (p) CTGGCCTCAGACCAG (VIC/MGB) |
75 | 62 | NC_006101.3 |
| iNOS | (f) GAGTGGTTTAAGGAGTTGGATCTGA
(r) TCCAGACCTCCCACCTCAAG (p) CTCTGCCTGCTGTTGCCAACATGCT (VIC/MGB) |
103 | 80 | NC_006106.3 |
| LITAF | (f) TCTGAGACCCCCAAGTCCAA
(r) CCTTAAGTTTTGCCAGAGGAGGTT (p) CCCACCACACCCACT (VIC/MGB) |
98 | 197 | NC_006104.3 |
IL-1β = interleukin 1 beta; IL-6 = interleukin 6; iNOS = inducible nitric oxide synthase; LITAF = lipopolysaccharide-induced TNF- α factor [43]; TLR-4 = toll-like receptor 4. f = forward primer; r = reverse primer; p = probe