. 2015 Oct 23;10(10):e0141215. doi: 10.1371/journal.pone.0141215

Table 4. The effect of thermally cool perch on mRNA expression of cytokines, toll-like receptors, and inducible nitric oxide synthase in the spleen of 32 week-old laying hens ¹ .

Treatment	IL-1β	IL-6	LITAF	TLR-4	iNOS
Cool Perch	0.75	0.79	1.65	2.35	1.33
Air Perch	0.69	0.60	1.87	2.40	1.30
No Perch	0.68	0.59	2.10	2.04	1.04
SEM	0.11	0.15	0.57	0.56	0.27
n ²	12	12	12	12	12
P-value	0.89	0.59	0.46	0.89	0.71

¹Data were presented as Mean±SEM. The data were calculated by the equation: tested mRNA quantity value (mean of replicated target mRNA value/GAPDH quantity mean of replicate endogenous control value).

²Average number of observations per least square mean.

GAPDH = glyceraldehyde 3-phosphate dehydrogenase; iNOS = inducible nitric oxide synthase; IL-1β = interleukin 1 beta; IL-6 = interleukin 6; TLR-4 = toll-like receptor 4; LITAF = lipopolysaccharide-induced TNF-α factor [43].

Table 4. The effect of thermally cool perch on mRNA expression of cytokines, toll-like receptors, and inducible nitric oxide synthase in the spleen of 32 week-old laying hens 1 .

Table 4. The effect of thermally cool perch on mRNA expression of cytokines, toll-like receptors, and inducible nitric oxide synthase in the spleen of 32 week-old laying hens ¹ .