Fig 4. Ex vivo interferon-gamma ELISPOT Responses to Heat killed Burkholderia pseudomallei by diabetes status.
Ex vivo IFN-γ ELIspot responses to heat-inactivated B. pseudomallei are shown for subjects with melioidosis on admission (Week 0) and 12 (Week12) and 52 (Week 52) weeks later according to the presence (+) or absence (-) of a diagnosis of Diabetes Mellitus. Peripheral blood mononuclear cells (PBMC) were stimulated with heat-inactivated B. pseudomallei for 18 hours and IFN-γ secreting cells counted and expressed as spot forming cells per million PBMC (SFC/106 PBMC). Subjects with diabetes had lower responses to B. pseudomallei during acute illness (Week 0) compared to subjects with no diabetes. Boxplots with Tukey whiskers are shown with responses greater than 1000 SFC/million PBMC not displayed. ** P < 0.01, ns = not significant by Kruskal-Wallis testing with Dunn’s multiple comparisons test.