Fig 4. Dynamics changes upon fibrinogen binding to thrombin.

Residue-wise mapping of flexibility difference maps Thr_com,Fib—Thr_holo,Fib with colors ranging from blue (complex more rigid than holo) over white (no change) to red (complex more flexible than holo). Rigidification on substrate binding is shown for global backbone B-factors (A). For φ entropy (B) rigidification of some parts on complex formation is shown. A slight mobilization of Leu-99 is observed. For ψ entropy (C), no significant binding site rigidification apart from the S1 and S2 sub-pocket is shown.