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WY-14643 pretreatment and sirtuins protein expression and activity in steatotic livers after ischemia-reperfusion. Western blot and densitometric analysis of (a) SIRT1 and (b) SIRT3. (c) SIRT1 enzymatic activity. Sham: anesthesia and laparotomy, IR: 60 min partial ischemia and 24 h of reperfusion, and WY-14643 + IR: iv administration of WY-14643 (10 mg/kg) 1 hour before IR. ∗ P < 0.05 versus Sham; # P < 0.05 versus IR.
