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. 2015 Oct 23;10(10):e0140781. doi: 10.1371/journal.pone.0140781

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© 2015 Slemmons et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — Cell proliferation, as measured by BrdU incorporation, is decreased in (A) RD and (B) SMS-CTR cells stably expressing YAP shRNAs. Immunoblot for cleaved caspase 3 (CC3) shows an increase in apoptosis at days 2 and 4 after shRNA expression in (C) RD and (D) SMS-CTR cells. qRT-PCR for YAP target genes Cyr61 and CTGF show decreased expression with YAP suppression in (E) RD and (F) SMS-CTR cells. Immunoblot analysis of TAZ in (G) RD and (H) SMS-CTR cells expressing YAP shRNAs. *, P< 0.05; **, P<0.01; and ****, P<0.0001.