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. 2015 Oct 11;2015:804260. doi: 10.1155/2015/804260

Figure 3.

Figure 3

Protein phosphatase 2A is activated by oxidative stress via AKT/mTOR inactivation in osteoblasts. (a) Biochemical assays indicate that PP2A phosphatase activity was induced by 4-HNE treatment in osteoblasts. (b) Western blots showed that protein levels of PP2A three subunits were not altered by 4-HNE treatment, indicating that 4-HNE activates PP2A not by increasing its protein levels. (c) Western blots showed that AKT/mTOR pathways, indicated by pAKT and pp70S6K, are upregulated by 4-HNE treatment, which may cause PP2A activation. Results are averages of three independent experiments. Data represent mean ± SEM. ∗∗ P < 0.01 and ∗∗∗ P < 0.001.