Table 1.
List of primer sequences
| Genes name | Forward primer | Tm(°C) | Length(bp) |
|---|---|---|---|
| Reverse primer | |||
| Oct4 | TTGAGGCTCTGCAGCTTAG | 60 | 285 |
| GCCGGTTACAGAACCACAC | |||
| Sox2 | ACACCAATCCCATCCACACT | 60 | 224 |
| GCAAACTTCCTGCAAAGCTC | |||
| Nanog | CCTGATTCTTCCACCAGTCC | 60 | 292 |
| TGCTATTCTTCGGCCAGTTG | |||
| Lin28 | ACCGGACCTGGTGGAGTATT | 60 | 204 |
| CTTCAGCGGACATGAGGCTA | |||
| E-cadherin | CGCATTGCCACATACACTCT | 60 | 252 |
| TTGGCTGAGGATGGTGTAAG | |||
| N-cadherin | AGTCAACTGCAACCGTGTCT | 60 | 337 |
| AGCGTTCCTGTTCCACTCAT | |||
| vimentin | GAGCTGCAGGAGCTGAATG | 60 | 344 |
| AGGTCAAGACGTGCCAGAG | |||
| α -SMA | CTGACTGAGCGTGGCTATTC | 58 | 452 |
| CCACCGATCCAGACAGAGTA | |||
| FAP | ATAGCAGTGGCTCCAGTCTC | 59 | 278 |
| GATAAGCCGTGGTTCTGGTC | |||
| Slug | CCTGGTTGCTTCAAGGACAC | 60 | 395 |
| TCCATGCTCTTGCAGCTCTC | |||
| snail | GGTTCTTCTGCGCTACTGCT | 59 | 285 |
| TAGGGCTGCTGGAAGGTAAA | |||
| twist | GTCCGCAGTCTTACGAGGAG | 60 | 294 |
| TGGAGGACCTGGTAGAGGAA | |||
| β-actin | CACGAAACTACCTTCAACTCC | 56 | 265 |
| CATACTCCTGCTTGCTGATC |