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Relative expression of TLR4 in primary mouse intrahepatic biliary epithelial cells stimulated by different concentrations (10 μg/ml, 20 μg/ml and 40 μg/ml) of Clonorchis sinensis excretory/secretory products (ESP) using quantitative real-time PCR. Each value represents the mean ± SE of three or more independent experiments. *means significant differences (P < 0.05) were found, compared with negative group
