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. Author manuscript; available in PMC: 2015 Oct 25.
Published in final edited form as: Cell Rep. 2015 Jul 2;12(2):244–257. doi: 10.1016/j.celrep.2015.06.024

Figure 1. CCL2 Levels Increase with Neoplastic Progression in Human and Mouse Colitis-Associated CRC.

Figure 1

(A) CCL2 expression levels in 11 human colitis-associated colorectal dysplasia and adenocarcinoma (ACA) cases. Left panel: total CCL2+ area from five fields (objective 40×) plotted for each case. Areas for quantification were no colitis (normal), colitis (no dysplasia/ACA), dysplasia, and ACA. Symbols are color-coded to track samples. Right panel: representative images of CCL2 immunostaining in human colon samples. Yellow triangles, epithelial cells; red triangles, infiltrating immune cells. Scale bar, 50 μm.

(B) CCL2 colonic protein levels from T-bet−/− Rag2−/− mice across the neoplastic continuum, quantitated by ELISA. Mean ± SEM with age and number of mice per group shown.

(C) Prevalence of dysplasia and neoplasia in Ccl2−/− T-bet−/− Rag2−/− and T-bet−/− Rag2−/− mice monitored by mouse age. Symbols represent data from individual mice, with color-coding indicating age in months.

*p < 0.05, **p < 0.01, and ***p < 0.001 (unpaired, two-tailed Student's t test). See also Figure S1.